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Figure 3 | Veterinary Research

Figure 3

From: SeMet attenuates OTA-induced PCV2 replication promotion by inhibiting autophagy by activating the AKT/mTOR signaling pathway

Figure 3

Effects of OTA treatment on autophagy in PCV2-infected PK-15 cells. A PK-15 cells were infected with or without PCV2 for 24 h then incubated in the presence or absence of 0.1 μM OTA for 48 h. After harvest, the expressions of LC3, ATG5 and β-actin were analyzed by Western blotting as described in Materials and methods. Data are presented as mean ± SE of three independent experiments. Significance compared with control, *P < 0.05 and **P < 0.01. Significance compared with PCV2, #P < 0.05 and ##P < 0.01. B PK-15 cells were transfected with GFP-LC3 plasmid. After 12 h, PK-15 cells were infected with or without PCV2 for 24 h then incubated in the presence or absence of 0.1 μM OTA for 48 h and the fluorescence signals were visualized by confocal immunofluorescence microscopy (Scale bar: 10 μm). The average number of LC3 puncta in each cell was determined from at least 100 cells in each group. Data are presented as mean ± SE of three independent experiments. Significance compared with control, *P < 0.05 and **P < 0.01. Significance compared with PCV2, #P < 0.05 and ##P < 0.01.

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