A hybrid protein containing MSP1a repeats and Omp7, Omp8 and Omp9 epitopes protect immunized BALB/c mice against anaplasmosis

Table 1 Comparison between signals and leukogram data between MSP1a/OMP7/8/9 protein-immunized mice and controls mice

	Immunized	Adjuvant	Non-immunized	p	Reference
Symptoms, animals with symptom (Total of animals)^a
Opaque fur	0 (5)	5 (5)	5 (5)	nd	0 (5)
Ruffled fur	0 (5)	5 (5)	5 (5)	nd	0 (5)
Weight loss	0 (5)	0 (5)	5 (5)	nd	0 (5)
Lethargy	0 (5)	5 (5)	5 (5)	nd	0 (5)
Death	0 (5)	0 (5)	2 (5)	nd	0 (5)
Leukogram^b
Leucocytes	5600.0 (529.2)*	7266.7 (1814.8)	7733.3 (1222.0)*	0.0415	3483.3 (2119.7)
Lymphocytes	2053.5 (835.3)	2640.0 (254.4)	2242.7 (671.0)	0.3967	2223.4 (778.2)
Monocytes	130.0 (29.5)*	180.7 (112.9)	253.3 (12.2)*	0.0071	210.7 (27.2)
Neutrophils	3318.7 (370.1)*	4457.3 (1411.7)	5104.0 (595.9)*	0.0380	2014.0 (1396.4)
Red cells, millions/mm³	5.6 (0.5)	5.5 (0.9)	6.4 (0.6)	0.0631	5.6 (0.6)
Hemoglobin g/dL	16.8 (1.4)	16.7 (2.8)	19.5 (1.9)	0.1324	16.9 (1.8)
Hematocrit %	50.3 (4.0)	50.3 (8.3)	58.7 (5.8)	0.1303	50.7 (5.5)
MCV/mm³	90.3 (0.6)	90.7 (0.6)	91.0 (0.0)	0.2605	90.3 (0.6)
MHV/mm³	30.0 (0.0)	30.0 (0.0)	30.0 (0.0)	0.9999	30.0 (0.0)
MCHC/mm³	33.0 (0.0)	33.0 (0.0)	33.0 (0.0)	0.9999	33.0 (0.0)
Platelets, millions/mm³	323.7 (97.0)	403.3 (139.5)	281.3 (138.6)	0.6447	309.7 (45.6)
Creatinine, mg/dL	1.2 (0.5)	1.2 (0.4)	1.2 (0.5)	0.9854	1.1 (0.5)
Urea, mg/dL	56.3 (33.6)	55.0 (8.9)	52.0 (18.5)	0.9999	61.3 (20.4)

nd: not done.
* significance found by Kruskal Wallis T-test.
^aAnimals with symptom (Total of animals).
^bFor leukogram data, only three animals have been used in each parameter. Mean (standard deviation).

ISSN: 1297-9716