Figure 1From: A hybrid protein containing MSP1a repeats and Omp7, Omp8 and Omp9 epitopes protect immunized BALB/c mice against anaplasmosisDesign of MSP1a/OMP7/8/9 protein. A The amino acid sequence of the MSP1a/OMP7/8/9 protein containing MSP1a repeats and two common sequences from OMP7, OMP8 and OMP9. The synthetic DNA was designed using a sequence (GenBank: JN564640.1) from the Anaplasma marginale St. Maries strain. B The 3D structure of the MSP1a/OMP7/8/9 protein was predicted using the i-TASSER algorithm [33]. According to the 3D prediction, the MSP1a repeats and two common sequences were exposed on the surface of the recombinant protein. C Each panel shows in detail the secondary structure of the MSP1a repeats (left panel) and two common sequences (middle and right panels).Back to article page