Infection model of M. bovis in Bomac cells. Time points 0, 6 and 54 h post-infection with an MOI between 2 and 10 are shown. Black columns correspond to strain JF4278 (co-culture), while grey columns correspond to strain L22/93 (co-culture). Vertically striped columns correspond to strain JF4278 alone in cell medium, while horizontally striped columns correspond to strain L22/93 alone in cell medium. The x-axis represents the number of hours post-infection (Bomac: Bomac cells free of BVDV; Bomac + BVDV: Bomac infected with BVDV), while y-axis represents the log10 CFU/well. The data shown are the mean values of triplicates of three independent experiments. Standard deviations of individual measurements are indicated as vertical bars. *P < 0.05, **P < 0.01, ***P < 0.001.