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Figure 2 | Veterinary Research

Figure 2

From: Recombinant chicken interleukin-7 as a potent adjuvant increases the immunogenicity and protection of inactivated infectious bursal disease vaccine

Figure 2

Identification of chIL-7 expression and its biological activity. A Identification of chIL-7 protein in different fractions by 10% SDS-PAGE and Coomassie blue staining. M, protein markers; lane 1, whole cell fraction from empty vector-transformed bacteria; lane 2, whole cell fraction from pET20b-chIL-7/H vector-transformed bacteria; lane 3, insoluble fraction; lane 4, cytoplasmic fraction; lane 5, periplasmic fraction; lane 6, purified chIL-7 from periplasmic fraction with Ni–NTA agarose beads. B Identification of chIL-7 protein in different fractions detected by Western blot. Lanes 1–6, the samples corresponding to A. C Identification of chIL-7 biological activity based on its ability to stimulate proliferation of 2E8 cells measured by the MTT assay.

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