Role in virulence of phospholipases, listeriolysin O and listeriolysin S from epidemic Listeria monocytogenes using the chicken embryo infection model

Table 2 Mean time until death of chicken embryos

L. monocytogenes genotype	BUG strain	Time (h) until 100% death^a	Time (h) until death of at least 50%^b	Number of eggs	Number of experiments
EGDe	BUG 1600	41.3 ± 1.35	29.8 ± 10.15	14	2
F2365	BUG 3012	36.5 ± 4.46	31.4 ± 4.35	26	5
F2365 ΔplcA	BUG 3673	41.3 ± 1.35	41.3 ± 1.35	13	2
F2365 Δhly	BUG 3671	–	–	21	4
F2365 ΔplcB	BUG4077	44.0 ± 0	32.0 ± 0	20	2
F2365 ΔllsA	BUG 3781	31.3 ± 1.89	29.3 ± 4.11	15	3
F2365 pHELP::llsA	BUG 3817	30.0 ± 0	24.0 ± 0	10	2
F2365 prfA*	BUG 3651	25.4 ± 3.7	17.1 ± 2.6	9	2
F2365 prfA* ΔplcA	BUG 3702	–	–	10	2
F2365 prfA* Δhly	BUG 3657	–	–	19	3
F2365 prfA* ΔplcB	BUG 3703	41.3 ± 7.9	19.3 ± 4.8	13	2

The number of eggs and number of independent experiments are shown.
^aMean time (hours) until complete (100%) death of chicken embryos infected with different L. monocytogenes strains.
^bMean time (hours) until death of at least 50% of the chicken embryos infected with different L. monocytogenes strains.

ISSN: 1297-9716