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Figure 2 | Veterinary Research

Figure 2

From: Role in virulence of phospholipases, listeriolysin O and listeriolysin S from epidemic Listeria monocytogenes using the chicken embryo infection model

Figure 2

LIPI-1 and LIPI-3 gene expression in a PrfA* background and survival curves of chicken embryos infected with different strains of L. monocytogenes F2365 PrfA*. A LIPI-1 and LIPI-3 genes transcript levels monitored by real-time qPCR in L. monocytogenes F2365 (WT) and F2365 PrfA* strain (PrfA*) strains grown to stationary phase (OD600 ≈ 3) in BHI in aerobic shaking conditions. *** = P < 0.001; **** = P < 0.0001. B Survival curves of 9-day old chicken embryos infected in the allantoic cavity with 5 × 102 L. monocytogenes of the indicated strains. The survival of the chicken embryos was followed for 48 h after infection by candling and embryo movement. Data from one representative experiment is shown. At least 2 independent experiments were performed (see Table 2 for details). C LIPI-1 genes mRNA levels examined by real-time qPCR in L. monocytogenes F2365 PrfA* (PrfA*) and in the L. monocytogenes F2365 PrfA* ΔplcA (PrfA* ΔplcA) strains grown to stationary phase OD600 ≈ 3 in BHI in aerobic shaking conditions. ** = P < 0.01. D LLO protein levels detected by Western blot. Levels of a loading control protein, EF-TU, are shown for comparison. Protein extracts of bacteria were prepared from L. monocytogenes F2365 PrfA* and PrfA* ΔplcA strains grown to stationary phase OD600 ≈ 3 in BHI in aerobic shaking conditions. E Assessment of the hemolytic activity of L. monocytogenes F2365 PrfA*, F2365 PrfA* Δhly and F2365 PrfA* ΔplcA in Trypcase Soy Agar + 5% Horse Blood after incubation at 37 °C during 24 h.

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