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Table 2 Detailed results of immunohistochemical analyses for ileal Peyer’s patch samples

From: Detection of PrPBSE and prion infectivity in the ileal Peyer’s patch of young calves as early as 2 months after oral challenge with classical bovine spongiform encephalopathy

Months pi Animal ID Immunohistochemistry (IHC) of ileal Peyer’s patch
No. pos. foll./ Total no. foll. No. pos. foll. In % TBM FDC ENS
0 (n = 2) (1 week pi) WAIT 17 0/3288 0
WAIT 18 0/3636 0
2 (n = 2) WAIT 15 0/3837 0
WAIT 16 0/5136 0
Ileocaecal junction (ICJ): ICJ: − ICJ: − ICJ: + s.pl.*
0/887 0
4 (n = 6) WAIT 11 2/1976 0.10 Fine granular (+)
WAIT 12 3/1820 0.16 Punctate/fine granular
WAIT 13 24/2873 0.84 Fine/coarse granular (+)
WAIT 14 19/1993 0.95 Fine/coarse granular (+)
WAIT 19 1*/3986 inconcl. Fine granular
WAIT 20 89/2088 4.26 Fine/coarse granular +
6 (n = 6) WAIT 05 25/2283 1.10 Fine/coarse granular +
WAIT 06 43/1997 2.15 Fine/coarse granular
WAIT 07 7/1783 0.39 Fine granular
WAIT 08 85/1622 5.24 Fine/coarse granular +
WAIT 09 6/1473 0.41 Punctate/fine granular
WAIT 10 1/2207 0.05 Punctate/fine granular
8 (n = 2) WAIT 02 0/2203 0
WAIT 03 17/1446 1.18 Fine/coarse granular
8 (n = 2) WAKT 01 0/3925 0
WAKT 02 0/2911 0
35 (n = 1) WAIT 04 9/184† 4.89 Fine/coarse granular
36 (n = 1) WAIT 01 8/131† 6.11 Coarse granular + ++ m.pl.
  1. pi: post-infection; No.: number; foll.: follicles; inconcl.: inconclusive; *: only seen with mAb 6C2; †: in one level of the block; TBM: tingible body macrophages; −: negative; FDC: follicular dendritic cells; (+): weak net-like staining pattern; +: clear dendritic network; −: negative; ENS: enteric nervous system, positive labelling in submucosal (s.pl.) or myenteric (m.pl.) plexus; +: 0–4 positive foci, ++: 5–10 positive foci; −: negative.