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Table 2 Detailed results of immunohistochemical analyses for ileal Peyer’s patch samples

From: Detection of PrPBSE and prion infectivity in the ileal Peyer’s patch of young calves as early as 2 months after oral challenge with classical bovine spongiform encephalopathy

Months pi

Animal ID

Immunohistochemistry (IHC) of ileal Peyer’s patch

No. pos. foll./

Total no. foll.

No. pos. foll.

In %

TBM

FDC

ENS

0 (n = 2)

(1 week pi)

WAIT 17

0/3288

0

WAIT 18

0/3636

0

2 (n = 2)

WAIT 15

0/3837

0

WAIT 16

0/5136

0

Ileocaecal junction (ICJ):

ICJ: −

ICJ: −

ICJ: + s.pl.*

0/887

0

4 (n = 6)

WAIT 11

2/1976

0.10

Fine granular

(+)

WAIT 12

3/1820

0.16

Punctate/fine granular

WAIT 13

24/2873

0.84

Fine/coarse granular

(+)

WAIT 14

19/1993

0.95

Fine/coarse granular

(+)

WAIT 19

1*/3986

inconcl.

Fine granular

WAIT 20

89/2088

4.26

Fine/coarse granular

+

6 (n = 6)

WAIT 05

25/2283

1.10

Fine/coarse granular

+

WAIT 06

43/1997

2.15

Fine/coarse granular

WAIT 07

7/1783

0.39

Fine granular

WAIT 08

85/1622

5.24

Fine/coarse granular

+

WAIT 09

6/1473

0.41

Punctate/fine granular

WAIT 10

1/2207

0.05

Punctate/fine granular

8 (n = 2)

WAIT 02

0/2203

0

WAIT 03

17/1446

1.18

Fine/coarse granular

8 (n = 2)

WAKT 01

0/3925

0

WAKT 02

0/2911

0

35 (n = 1)

WAIT 04

9/184†

4.89

Fine/coarse granular

36 (n = 1)

WAIT 01

8/131†

6.11

Coarse granular

+

++ m.pl.

  1. pi: post-infection; No.: number; foll.: follicles; inconcl.: inconclusive; *: only seen with mAb 6C2; †: in one level of the block; TBM: tingible body macrophages; −: negative; FDC: follicular dendritic cells; (+): weak net-like staining pattern; +: clear dendritic network; −: negative; ENS: enteric nervous system, positive labelling in submucosal (s.pl.) or myenteric (m.pl.) plexus; +: 0–4 positive foci, ++: 5–10 positive foci; −: negative.