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Table 1 Results of ileal Peyer’s patch samples with reference to BSE status and Obex IHC results

From: Detection of PrPBSE and prion infectivity in the ileal Peyer’s patch of young calves as early as 2 months after oral challenge with classical bovine spongiform encephalopathy

Months pi Animal ID Status Obex (IHC) Ileal Peyer’s patch
IHC PMCA Tgbov XV mouse bioassay#
0 (n = 2) (= 1 week pi) WAIT 17 Preclinical neg. neg. 7/19, 567 ± 43
WAIT 18 Preclinical neg. neg. 2/21, 424 ± 76
2 (n = 2) WAIT 15 Preclinical neg. neg. + 12/20, 537 ± 42
WAIT 16 Preclinical neg. neg. 3/21, 525 ± 47
ICJ: +s.pl.* ICJ: −
4 (n = 6) WAIT 11 Preclinical neg. pos. 3/20, 567 ± 58
WAIT 12 Preclinical neg. pos. + (+) 17/20, 396 ± 28 (~)
WAIT 13 Preclinical neg. pos. + 14/20, 449 ± 19 (~)
WAIT 14 Preclinical neg. pos. ++ 20/20, 362 ± 12
WAIT 19 Preclinical neg. inconcl. + 10/20, 473 ± 17 (~)
WAIT 20 Preclinical neg. pos. ++ (+) 20/20, 315 ± 11
6 (n = 6) WAIT 05 Preclinical neg. pos. ++ 19/20, 307 ± 15
WAIT 06 Preclinical neg. pos. ++ 19/20, 368 ± 16
WAIT 07 Preclinical neg. pos. + (+) 19/20, 383 ± 15 (~)
WAIT 08 Preclinical neg. pos. ++ (+) 20/20, 319 ± 8
WAIT 09 Preclinical neg. pos. ++ 20/20, 366 ± 14
WAIT 10 Preclinical neg. pos. + 19/20, 383 ± 16
8 (n = 2) WAIT 02 Preclinical neg. neg. ++ (+) 18/20, 401 ± 21
WAIT 03 Preclinical neg. pos. +++ 18/20, 396 ± 12
8 (n = 2) WAKT 01 Negative control neg. neg. 0/20, > 730
WAKT 02 Negative control neg. neg. 0/20, > 730
35 (n = 1) WAIT 04 3 (Positive control) pos. pos. +++ Not done
36 (n = 1) WAIT 01 2 (Positive control) pos. pos. ++ (+) Not done
  1. pi: post infection; ICJ: ileocaecal junction; IHC: immunohistochemistry; neg.: negative; pos.: positive; inconcl.: inconclusive; +s.pl.*: positive submucosal plexus (only seen with mAb 6C2); PMCA: protein misfolding cyclic amplification; +: positive in the third round; ++: positive in the second and third round; +++: positive from the first round; (+): weak positive; −: negative; # positive/inoculated mice and mean incubation time in days ± standard error of the mean (SEM); (~) bioassay is ongoing: so far positive/inoculated mice and their mean incubation time in days ± standard error of the mean (SEM).