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Table 1 Strains and plasmids

From: Salmonella enterica serovar Choleraesuis vector delivering SaoA antigen confers protection against Streptococcus suis serotypes 2 and 7 in mice and pigs

Strain or plasmid Relevant characteristics or genotype Source or references
E. coli strains
 BL21 F pT hsdSB(rB mB al dcm (DE3) Invitrogen
 χ7213 thi-1 thr-1 leuB6 fhuA21 lacY1 glnV44 asdA4 recA1 RP4 2-Tc::Mu pir; Km r Dr Curtiss gift
Salmonella Choleraesuis
 C78-3 Wild type, virulent, CVCC79103 [65]
 C500 Salmonella Choleraesuis vaccine strain attenuated by chemical mutation, CVCC79500 [37]
 rSC0010 ΔPcrp527::TT araC PBAD crp Δpmi-2426 ΔrelA199::araC PBAD lacI TT [34]
 rSC0011 ΔPcrp527::TT araC PBAD crp Δpmi-2426 ΔrelA199::araC PBAD lacI TT ΔasdA33 [34]
 rSC0013 rSC0010 ΔsopB1686 This work
 rSC0016 rSC0013 ΔasdA33 This work
 rSC0017 C78-3 ΔsopB1686 This work
 rSC0018 C500 ΔasdA33 This work
 Streptococcus suis serotype 2 Wild-type, virulent, CVCC3928 Lab stock
 Streptococcus suis serotype 7 Wild-type isolated in field Lab stock
Plasmids
 pYA3493 Plasmid Asd + ; pBR ori, β-lactamase signal sequence-based periplasmic secretion plasmid [46]
 pET28a expression vector, T7 promoter; Kmr Novagen
 pRE112 oriT oriV sacB CmR [34]
 pS006 suicide vector for ΔsopB1686 This work
 pYA3736 suicide vector for ΔasdA33 pRE112
 pET28a-saoA expression vector for purification of Hig-tag SaoA, T7 promoter, Km+ pET28a
 pS-SaoA pYA3493 with SaoA pYA3493