E. coli strains
|
BL21
|
F−
pT hsdSB(rB− mB−
al dcm (DE3)
|
Invitrogen
|
χ7213
|
thi-1 thr-1 leuB6 fhuA21 lacY1 glnV44 asdA4 recA1 RP4 2-Tc::Mu pir; Km
r
|
Dr Curtiss gift
|
Salmonella Choleraesuis
|
C78-3
|
Wild type, virulent, CVCC79103
|
[65]
|
C500
|
Salmonella Choleraesuis vaccine strain attenuated by chemical mutation, CVCC79500
|
[37]
|
rSC0010
|
ΔPcrp527::TT araC PBAD
crp Δpmi-2426 ΔrelA199::araC PBAD
lacI TT
|
[34]
|
rSC0011
|
ΔPcrp527::TT araC PBAD
crp Δpmi-2426 ΔrelA199::araC PBAD
lacI TT ΔasdA33
|
[34]
|
rSC0013
|
rSC0010 ΔsopB1686
|
This work
|
rSC0016
|
rSC0013 ΔasdA33
|
This work
|
rSC0017
|
C78-3 ΔsopB1686
|
This work
|
rSC0018
|
C500 ΔasdA33
|
This work
|
Streptococcus suis serotype 2
|
Wild-type, virulent, CVCC3928
|
Lab stock
|
Streptococcus suis serotype 7
|
Wild-type isolated in field
|
Lab stock
|
Plasmids
|
pYA3493
|
Plasmid Asd + ; pBR ori, β-lactamase signal sequence-based periplasmic secretion plasmid
|
[46]
|
pET28a
|
expression vector, T7 promoter; Kmr
|
Novagen
|
pRE112
|
oriT oriV sacB CmR
|
[34]
|
pS006
|
suicide vector for ΔsopB1686
|
This work
|
pYA3736
|
suicide vector for ΔasdA33
|
pRE112
|
pET28a-saoA
|
expression vector for purification of Hig-tag SaoA, T7 promoter, Km+
|
pET28a
|
pS-SaoA
|
pYA3493 with SaoA
|
pYA3493
|