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Table 2 Detection of hPPMV-1/Netherlands/579/2003 by qRT-PCR (threshold cycle value) and virus isolation (TCID 50 /mL) in respiratory tract organs and tracheo-bronchial lymph nodes of cynomolgus macaques at 3 dpi

From: Pigeon paramyxovirus type 1 from a fatal human case induces pneumonia in experimentally infected cynomolgus macaques (Macaca fascicularis)

Macaque no.

Nasal concha

Trachea

Bronchus

Upper lung lobe

Lower lung lobe

Broncho-alveolar lavage fluid

Tracheo-bronchial lymph node

qRT-PCR

VI

qRT-PCR

VI

qRT-PCR

VI

qRT-PCR

VI

qRT-PCR

VI

qRT-PCR

VI

qRT-PCR

VI

1

−

nd

34.9

–

35.8

–

–

nd

29.7

1.2 × 103

25.1

nd

–

nd

2

36.4

–

37.0

–

37.3

–

38.8

1.6 × 101

27.2

8.4 × 101

22.8

nd

–

nd

3

32.2

–

38.7

–

–

nd

29.7

–

32.2

1.3 × 103

23.3

nd

34.2

–

  1. Adrenal gland, brain, cerebrospinal fluid, conjunctiva, heart, kidney, mesenteric lymph node, large intestine, liver, nasal septum, pancreas, small intestine, and spleen tested negative by qRT-PCR for PPMV-1.
  2. VI: virus isolation; −: negative; nd: not done.