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Table 2 Responses to F, H and NP peptides in PPRV IC’89-infected sheep

From: Vaccination with recombinant adenovirus expressing peste des petits ruminants virus-F or -H proteins elicits T cell responses to epitopes that arises during PPRV infection

Peptide ELISPOT (positive/total sheep) Intracellular IFN-γ staining Cytotoxicity assay
F3 2/8 CD4+ (n = 2) ND
F7 1/8 ND ND
F8 5/8 CD4+/CD8+ (n = 4) Not detected (n = 2)
F9 3/8 CD4+/CD8+ (n = 3) Not detected (n = 2)
F10 7/8 CD4+/CD8+ (n = 5) Positive (n = 2)
H4 2/8 CD8+ (n = 2) Positive (n = 1)
H6 2/8 CD4+ (n = 2) ND
H7 1/8 ND ND
H8 1/8 ND ND
H9 3/8 CD4+/CD8+ (n = 2) Positive (n = 1)
H10 3/8 CD8+ (n = 2) Positive (n = 1)
NP1 1/8 ND ND
NP3 1/8 ND ND
NP7 2/8 ND ND
NP8 3/8 CD4+/CD8+ (n = 2) Positive (n = 1)
NP9 3/8 CD4+/CD8+ (n = 2) Positive (n = 2)
NP10 1/8 ND ND
  1. Due to limited PBMC numbers it was not possible to use all three techniques described on all sheep PBMC.
  2. n: number of animal tested, ND: not done.