Table 2 Responses to F, H and NP peptides in PPRV IC’89-infected sheep
Peptide | ELISPOT (positive/total sheep) | Intracellular IFN-γ staining | Cytotoxicity assay |
|---|---|---|---|
F3 | 2/8 | CD4+ (n = 2) | ND |
F7 | 1/8 | ND | ND |
F8 | 5/8 | CD4+/CD8+ (n = 4) | Not detected (n = 2) |
F9 | 3/8 | CD4+/CD8+ (n = 3) | Not detected (n = 2) |
F10 | 7/8 | CD4+/CD8+ (n = 5) | Positive (n = 2) |
H4 | 2/8 | CD8+ (n = 2) | Positive (n = 1) |
H6 | 2/8 | CD4+ (n = 2) | ND |
H7 | 1/8 | ND | ND |
H8 | 1/8 | ND | ND |
H9 | 3/8 | CD4+/CD8+ (n = 2) | Positive (n = 1) |
H10 | 3/8 | CD8+ (n = 2) | Positive (n = 1) |
NP1 | 1/8 | ND | ND |
NP3 | 1/8 | ND | ND |
NP7 | 2/8 | ND | ND |
NP8 | 3/8 | CD4+/CD8+ (n = 2) | Positive (n = 1) |
NP9 | 3/8 | CD4+/CD8+ (n = 2) | Positive (n = 2) |
NP10 | 1/8 | ND | ND |