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Table 1 PPRV-F, -H and -NP peptide prediction and binding assays to H-2D b and H-2 K b molecules

From: Vaccination with recombinant adenovirus expressing peste des petits ruminants virus-F or -H proteins elicits T cell responses to epitopes that arises during PPRV infection

  PPRV protein (position) Sequence Predicted allele binding Score SYFPEITHI Score ProPred-I Score NetMHC (predicted affinity nM) Binding assay scorea
F1 F(53–61) KLMPNITAI Db 23 138.312 365.28 ++
F2 F(135–143) QSLMNSQAI Db 25 3281.040 34.37 ++
F3 F(369–377) GTTSNRFIL Db 19 286.272 3413.74 +
F4 F(342–350) NALYPMSPL Db 16 30.240 1927.14
Kb 18 0.330 791.56 +++
F5 F(435–443) SVYLHKIDL Kb 11 0.100 68.79 +
F6 F(278–286) IAYPTLSEI Db 18 4.234 3403.64 +
Kb 9 1.650 168.59 +
F7 F(7–21) LVFLFLFPNTVTCQI I-Ab NA NA 79.2 NA
F8 F(117–131) VALGVATAAQITAGV I-Ab NA NA 192.1 NA
F9 F(341–355) QNALYPMSPLLQECF I-Ab NA NA 351.5 NA
F10 F(284–298) LSEIKGVIVHKIEAI Homologue to morbillivirus epitope [38, 39] NA NA 5014.3 (I-Ab) NA
H1 H(270–278) FHMTNYLTV Db 21 17.280 43.88 +++
H2 H(158–166) AAVKSVEHI Db 21 50.168 593.13 +++
H3 H(547–555) RSSSYFYPV Kb 18 1.100 70.29 ++
H4 H(549–557) SSYFYPVRL Kb 18 5.500 8.75 +++
H5 H(551–559) YFYPVRLNF Db 7 0.000 42 098.88 +
Kb 7 0.120 1250.29
H6 H(44–52) VMFLSLIGL Db 15 14.334 6563.96 ++
Kb 11 2.000 44.36 +++
H7 H(426–434) VITSVFGPL Kb 21 12.000 96.10 +++
H8 H(441–455) MDLYNNPFSRAAWLA I-Ab NA NA 42.2 NA
H9 H(427–441) ITSVFGPLIPHLSGM I-Ab NA NA 74.8 NA
H10 H(448–462) FSRAAWLAVPPYEQS I-Ab NA NA 319.6 NA
NP1 NP(29–37) RGIKNVIIV Db 19 38.880 265.97 +++
NP2 NP(72–80) VMISMLSLF Db 18 36.318 3827.32
Kb 11 0.030 97.12 +++
NP3 NP(294–302) STIESLMNL Db 18 12.773 7155.04
Kb 12 1.320 117.94 ++
NP4 NP(335–343) YAMGVGVEL Db 16 30.240 6163.56 +
NP5 NP(354–362) RSYFDPAYF Db 11 0.091 4399.92
Kb 8 0.158 1703.38
NP6 NP(228–236) SLRRFMVSL Kb 12 0.264 184.41 ++
NP7 NP(298–306) SLMNLYQQL Kb 22 26.400 85.56 +
NP8 NP(435–449) REEVKAAIPNGSEGR I-Ab NA NA 95.2 NA
NP9 NP(327–341) GAYPLLWSYAMGVGV I-Ab NA NA 290.8 NA
NP10 NP(176–190) ILLAKAVTAPDTAAD I-Ab NA NA 355.6 NA
  1. NA: not available, +++ strong binder (ratio > 0.9), ++ moderate binder (0.7 < ratio < 0.9), + weak binder (0.5 < ratio < 0.7), no binder (ratio < 0.5).
  2. aPeptide score was ranked relative to LCMV gp33-41 peptide binding (ratio PPRV peptide MFI vs gp 33–41 MFI) [32].