Vaccination with recombinant adenovirus expressing peste des petits ruminants virus-F or -H proteins elicits T cell responses to epitopes that arises during PPRV infection

Table 1 PPRV-F, -H and -NP peptide prediction and binding assays to H-2D ^b and H-2 K ^b molecules

	PPRV protein (position)	Sequence	Predicted allele binding	Score SYFPEITHI	Score ProPred-I	Score NetMHC (predicted affinity nM)	Binding assay score^a
F1	F(53–61)	KLMPNITAI	D^b	23	138.312	365.28	++
F2	F(135–143)	QSLMNSQAI	D^b	25	3281.040	34.37	++
F3	F(369–377)	GTTSNRFIL	D^b	19	286.272	3413.74	+
F4	F(342–350)	NALYPMSPL	D^b	16	30.240	1927.14	−
F4	F(342–350)	NALYPMSPL	K^b	18	0.330	791.56	+++
F5	F(435–443)	SVYLHKIDL	K^b	11	0.100	68.79	+
F6	F(278–286)	IAYPTLSEI	D^b	18	4.234	3403.64	+
F6	F(278–286)	IAYPTLSEI	K^b	9	1.650	168.59	+
F7	F(7–21)	LVFLFLFPNTVTCQI	I-A^b	NA	NA	79.2	NA
F8	F(117–131)	VALGVATAAQITAGV	I-A^b	NA	NA	192.1	NA
F9	F(341–355)	QNALYPMSPLLQECF	I-A^b	NA	NA	351.5	NA
F10	F(284–298)	LSEIKGVIVHKIEAI	Homologue to morbillivirus epitope [38, 39]	NA	NA	5014.3 (I-A^b)	NA

NA: not available, +++ strong binder (ratio > 0.9), ++ moderate binder (0.7 < ratio < 0.9), + weak binder (0.5 < ratio < 0.7), − no binder (ratio < 0.5).
^aPeptide score was ranked relative to LCMV gp33-41 peptide binding (ratio PPRV peptide MFI vs gp 33–41 MFI) [32].

ISSN: 1297-9716