Ad5-F and Ad5-H vaccination produces functional memory T cells to PPRV immunogenic peptides. PBMC from Ad5-F- or Ad5-H-vaccinated sheep (21 days after booster and prior to PPRV challenge) were stimulated with PPRV immunogenic peptides and stained for CD4, CD8, the memory marker CD45RO and intracellular IFN-γ. A Mean (± SEM) IFN-γ production in CD45RO+ and CD45RO−
A CD4+ or B CD8+ T cells in two to four Ad5-F- or Ad5-H-immunized sheep is plotted. PMA and ionomycin was used as positive control. Representative IFN-γ and CD45RO stainings in A CD4+ or B CD8+ T cells from Ad5-F- or Ad5-H-vaccinated sheep in response to PPRV immunogenic peptide stimulation. Student’s t test (CD45RO+ vs CD45RO−); *p < 0.05. IFN-γ production in PBMC from C Ad5-F- or D Ad5-H-vaccinated sheep was measured pre-challenge and after PPRV IC’89 challenge by ELISPOT assays. Data were normalized to spots detected in unstimulated cells. p ≤ 0.1 in paired Student’s t tests are shown.