Table 3 Primers used in this study
Primer | Sequence (5′– > 3′) |
|---|---|
degS_left_for | GAATTCCTGCAGCCCGCGAACGGCTAAATCTATATGATG |
degS_left_rev | CCAAGGTTGAAACGAAACCTAGTGCAATCGCTTGTAC |
degS_right_for | TTGACGGAGGGCTTTGGCGAATTTCCGGAACTATAATGC |
degS_right_rev | ACTAGTGGATCCCCCCGCAACCTCACGATTTCTATCTC |
nlpI_left-for | GAATTCCTGCAGCCCGCGTGATGGAACAAGCGATTC |
nlpI_left-rev | CCAAGGTTGAAACGAGCATCAAGAAGCGAAGGTGAAAC |
nlpI_right-for | TTGACGGAGGGCTTTACGTGGTGGTAGCAATGTTG |
nlpI_right-rev | ACTAGTGGATCCCCCTTGTAGTGCAGAGAGGTCTAACG |
dfrA14-degS_for | CGATTGCACTAGGTTTCGTTTCAACCTTGGTGTTTGG |
dfrA14-degS_rev | TTCCGGAAATTCGCCAAAGCCCTCCGTCAAATTTATTACC |
dfrA14-nlpI_for | TTCGCTTCTTGATGCTCGTTTCAACCTTGGTGTTTGG |
dfrA14-nlpI_rev | TTGCTACCACCACGTAAAGCCCTCCGTCAAATTTATTACC |
degS_test | AATAATGACCGAACACATCC |
nlpI_test | ATTTCGTCCGCTTCATCC |
dfrA14_test-for | TCGTTTCAACCTTGGTGTTTGG |
dfrA14_test-rev | AAAGCCCTCCGTCAATTTTATTACC |
apfA_pET44_for | GTCCCaCGaGGaAGCCAgAAgCTAAGTCTTATTCGACCa |
apfA_pET44_rev | ACTTcATTAACATTAGTTTATCGCgCAGAAATTTGCC |
pET44_apfA_rev | AAGACTTAGcTTcTGGCTtCCtCGtGGGACCAG |
pET44_apfA_for | TTCTGcGCGATAAAcTAATGTTAATgAAGTTGGGCGTTCCT |
vacJ_pET44_for | GTCCCaCGaGGaAGCAAgTTAAAgCAATTAAGgTTAGTAGCC |
vacJ_pET44_rev | ACTTcATTAACATTAATCAATgTCTTTcAATTCTTCTTCGG |
pET44_vacJ_rev | TAATTGcTTTAAcTTGCTtCCtCGtGGGACCAG |
pET44_vacJ_for | TTgAAAGAcATTGATTAATGTTAATgAAGTTGGGCGTTCCT |