Strain/plasmids | Description | References |
---|---|---|
E. coli | ||
 JOL232 (χ6212) | F-λ-φ80 Δ(lacZYA-argF) endA1 recA1 hadR17 deoR thi-1 glnV44 gyrA96 relA1 ΔasdA4 | Lab stock |
Salmonella Typhimurium | ||
 JOL 401 | Wild type isolate from porcine, challenge strain | Lab stock |
 JOL1311 | Δasd, used as base vaccine strain | Lab stock |
 JOL1950 | JOL1311 containing pJHL464 | This study |
Plasmids | ||
 pJHL172 | asd + vector, pBR ori plasmid harboring cI857/λPR promoter, araC ParaBAD, phiX174 lysis gene E | [22] |
 pJHL319 | T-easy vector harboring E gene ghost cassette with the convergent promoter system | This study |
 pJHL360 | T-easy vector harboring the R ghost cassette with the convergent promoter system | This study |
 pJHL464 | asd+ vector, pBR ori plasmid harboring cI857/λPR promoter, araC P araBAD , the R ghost cassette composed of S, R and R1/Rz genes | This study |