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Table 1 Bacterial strains and plasmids utilized in this study

From: Salmonella Typhimurium, the major causative agent of foodborne illness inactivated by a phage lysis system provides effective protection against lethal challenge by induction of robust cell-mediated immune responses and activation of dendritic cells

Strain/plasmids Description References
E. coli
 JOL232 (χ6212) F-λ-φ80 Δ(lacZYA-argF) endA1 recA1 hadR17 deoR thi-1 glnV44 gyrA96 relA1 ΔasdA4 Lab stock
Salmonella Typhimurium
 JOL 401 Wild type isolate from porcine, challenge strain Lab stock
 JOL1311 Δasd, used as base vaccine strain Lab stock
 JOL1950 JOL1311 containing pJHL464 This study
Plasmids
 pJHL172 asd + vector, pBR ori plasmid harboring cI857/λPR promoter, araC ParaBAD, phiX174 lysis gene E [22]
 pJHL319 T-easy vector harboring E gene ghost cassette with the convergent promoter system This study
 pJHL360 T-easy vector harboring the R ghost cassette with the convergent promoter system This study
 pJHL464 asd+ vector, pBR ori plasmid harboring cI857/λPR promoter, araC P araBAD , the R ghost cassette composed of S, R and R1/Rz genes This study
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Veterinary Research

ISSN: 1297-9716