E. coli
|
JOL232 (χ6212)
|
F-λ-φ80 Δ(lacZYA-argF) endA1 recA1 hadR17 deoR thi-1 glnV44 gyrA96 relA1 ΔasdA4
|
Lab stock
|
Salmonella Typhimurium
|
JOL 401
|
Wild type isolate from porcine, challenge strain
|
Lab stock
|
JOL1311
|
Δasd, used as base vaccine strain
|
Lab stock
|
JOL1950
|
JOL1311 containing pJHL464
|
This study
|
Plasmids
|
pJHL172
|
asd
+ vector, pBR ori plasmid harboring cI857/λPR promoter, araC ParaBAD, phiX174 lysis gene E
|
[22]
|
pJHL319
|
T-easy vector harboring E gene ghost cassette with the convergent promoter system
|
This study
|
pJHL360
|
T-easy vector harboring the R ghost cassette with the convergent promoter system
|
This study
|
pJHL464
|
asd+ vector, pBR ori plasmid harboring cI857/λPR promoter, araC P
araBAD
, the R ghost cassette composed of S, R and R1/Rz genes
|
This study
|