In vitro analysis of dendritic cells differentiated by JOL1950 ghosts. Co-stimulatory molecule expression on the dendritic cell surface was assessed by gating on CD11c+ cell populations by FACS. A Microscopic images of DCs captured with an inverted microscope (magnification × 100). Representative micrographs of DC culture medium containing murine GM–CSF and IL-4 during 5 days, and DC cultures stimulated with JOL1950 ghosts for an additional 48 h (on the 7th day of culture). DCs differentiated by stimulation displayed morphological characteristics typical of DCs, such as the presence of dendritic processes, as indicated by the arrows. DCs supplemented with GM-CSF and IL-4 or medium only were round in appearance or displayed veils. B Representative FACS histogram of the surface marker-positive cell population after 48 h of stimulation with the ghost cells or LPS. C Proportion (%) of co-stimulatory molecule-positive DCs after 48 h of stimulation with the ghost cells or LPS. *P < 0.05 (vs. non-stimulated DCs). D Cytokine mRNA upregulation in DCs co-cultured with BG. *P < 0.05 (vs. cytokine mRNA upregulated in LPS-stimulated DCs).