Skip to main content

Table 3 Comparison of histopathological analysis and most abundant genera found by next generation sequencing (NGS) in available placenta samples

From: Amplicon sequencing of bacterial microbiota in abortion material from cattle

Sample ID Placentitis Necrosis Type of infiltrate Vasculitis Presence of ICBa Presence of ECBb Most abundant genera found by NGS (≤ 75% of the reads) Comments
12Ue1096_Pl Mild Mild Mixed Yes Yes Yes Pseudomonas (86.72%), others (13.28%)  
12Ue1503_Pl No Moderate No No No Yes Pseudomonas (91.26%), others (8.74%) Pseudomonas (99.68%) in the abomasal content
13Ue218_Pl No No No No No Yes Clostridium (29.94%), Enterococcus (9.71%), Ruminococcaceae a (9.67%), Flavobacterium (6.89%), Bacteroidales b (5.18%), Enterobacteriaceae (4.46%), Pseudomonas (3.32%), Succinivibrio (2.88%), RF16 (2.43%), others (22.63%)  
13Ue238_Pl NA NA NA NA NA NA Psychrobacter (30.22%), Shewanella (29.58%), Flavobacteriales b (12.76%), Pseudomonadaceae a (4.50%), others (22.94%) Severe autolysis
13Ue491_Pl Mild Moderate Mononuclear No No No Bacteroides (39.09%), Pseudomonas (26%), Shewanella (21.69%), others (13.22%)  
13Ue851_Pl Moderate Moderate Neutrophilic Yes No No Enterobacteriaceae a (46.46%), Enterobacteriales b (24.33%), Serratia (16.14%), others (13.07%)  
13Ue1008_Pl Moderate Moderate Mixed Yes No Yes Vibrio (89.92%), others (10.08%) V. metschnikovii isolated in culture
13Ue1137_Pl Moderate Moderate Mixed Yes No Yes Streptococcus (99.33%), others (0.67%) Streptococcus (99.62%) in the abomasal content,
S. uberis isolated in culture
13Ue1143_Pl Mild Mild Neutrophilic No No Yes Enterobacteriaceae a (96.19%), others (3.81%) Enterobacteriaceae a (99.17%) in the abomasal content. E. coli isolated in culture
13Ue1218_Pl Mild Mild Mixed Yes No Yes Clostridium (28.33%), Cetobacterium (13.16%), Enterobacteriaceae a (13.02%), Peptostreptococcaceae a (12.42%), Aeromonadaceae a (9.8%), others (23.27%) High level of contamination with plants and feces
13Ue1275_Pl Mild Mild Mixed Yes No No Streptococcus (37.24%), Enterobacteriaceae a (25.92%), Lactococcus (21.27%), others (15.57%) S. uberis isolated in pure culture
13Ue1631_Pl NA NA NA NA NA NA Pseudomonadales b (16.87%), Pseudomonadaceae a (16.24%), Propionibacteriaceae a (11.03%), Acinetobacter (9.13%), Aeromonadaceae a (6.81%), Candidatus Portiera (5.66%), Ruminococcaceae a (3.62%), Psychrobacter (3.33%), others (23.34%) Severe autolysis
13Ue1644_Pl No No No No No   Psychrobacter (83.99%), others (16.01%)  
13Ue1714_Pl NA NA NA NA NA NA Actinobacillus (38.74%), Clostridium (15.78%), Streptococcus (14.54%), Enterobacteriaceae a (7.83%), others (23.11%) Severe autolysis
13Ue1756_Pl NA NA NA NA NA NA Psychrobacter (33.24%), Pseudomonadaceae a (15.06%), Aeromonadaceae a (13.06%), Acinetobacter (12.23%), Vibrio (5.53%), others (20.88%) Severe autolysis
14A20_Pl Mild Mild Mixed No No Yes Aeromonadaceae a (73%), Pseudomonas (26.26%), others (0.74%)  
14A28_Pl NA NA NA NA NA NA Shewanella (24.04%), Camomonas (17.94%), Psychrobacter (9.97%), Pseudomonadaceae a (8.82%), Pseudomonas (4.49%), Acinetobacter (4.5%), Wautersiella (3.66%), Staphylococcus (3.64%), others (22.94%) Severe autolysis
  1. Cases with positive results for parasitology, virology and/or with presence of intralesional fungal organisms were not included.
  2. aPresence of intracytoplasmic bacteria (ICB).
  3. bPresence of extracellular bacteria (ECB).