Cross protective immune responses in nursing piglets infected with a US spike-insertion deletion porcine epidemic diarrhea virus strain and challenged with an original US PEDV strain

Table 1 Fecal consistency scores and fecal PEDV RNA shedding titers in original US PEDV-, S-INDEL PEDV-, and mock (control)-inoculated piglets post inoculation and after challenge with the original US PEDV virus

	Mean fecal PEDV RNA shedding titers (SD) (log₁₀ GE/mL)			Mean fecal consistency scores (SD)			Pig diarrhea rates (%)
	Control	S-INDEL	Original US PEDV	Control	S-INDEL	Original US PEDV	Control	S-INDEL	Original US PEDV
DPI 1–7	ND	8.2^b (1.1)	9.1^a (1.0)	0^c	1.7^b (1.0)	2.4^a (0.5)	0^b	100^a	100^a
DPI 8–14	ND	7.8 (1.1)	8.2 (0.2)	0	0.3 (0.5)	0.8 (0.9)	0^b	0^b	28^a
DPI 15–21	ND	6.7^b (0.9)	8.1^a (0.3)	0	0	0	0	0	0
DPC 1–7	8.7^a (0.8)	6.0^b (3.1)	6.2^b (0.3)	1.2^a (1.0)	1.1^a (0.9)	0^b	100^a	81^a	0^b

Fecal viral RNA shedding titers were determined by TaqMan real-time reverse transcription-PCR.
Fecal consistency scores 0, 1, 2 and 3 correspond to normal, pasty, semi-liquid, and liquid feces, respectively, with scores of ≥ 2 corresponding to diarrhea.
Values with different letters (a, b and c) differ significantly between groups (one-way ANOVA for means and Fisher’s Exact test for diarrhea rates, respectively. p < 0.05).
SD: standard deviation, ND: not detectable.

ISSN: 1297-9716