| |
Basis of diagnosis
|
Tissuea
|
Fluid
|
Faeces
|
|---|
|
Sensitivity % (n = positive/total)
|
RT-qPCR alone
|
89.8 (n = 202/225)
|
78.4 (n = 40/51)
|
64.6 (n = 31/48)
|
|
Combination testingb
|
80.9 (n = 182/225)
|
60 (n = 30/50)c
|
33.3 (n = 16/48)
|
|
Specificity % (n = negative/total)
|
RT-qPCR alone
|
92.6 (n = 239/258)
|
97.9 (n = 46/47)
|
80 (n = 28/35)
|
|
Combination testingb
|
94.6 (n = 244/258)
|
97.9 (n = 46/47)
|
100 (n = 35/35)
|
|
Accuracy % (n = true result/total)
|
RT-qPCR alone
|
91.3 (n = 441/483)
|
87.8 (n = 86/98)
|
71.1 (n = 59/83)
|
|
Combination testingb
|
88.2 (n = 426/483)
|
78.4 (n = 76/97)
|
61.4 (n = 41/83)
|
- The reference-standard for diagnosis of FIP was considered identification of FCoV antigen by immunohistochemistry in at least one tissue in association with appropriate histopathological changes, and cats were considered “without FIP” where FIP was excluded as a diagnosis.
-
aAs some positive samples without histopathological data were not subjected to sequencing, only those samples with histopathological data available were included in these calculations.
-
bRT-qPCR in combination with spike protein sequence characterisation FCoV.
-
cOne sample lost from analysis.
