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Table 5 Sensitivity, specificity and accuracy of diagnosis of FIP using molecular diagnostics

From: Limitations of using feline coronavirus spike protein gene mutations to diagnose feline infectious peritonitis

  Basis of diagnosis Tissuea Fluid Faeces
Sensitivity % (n = positive/total) RT-qPCR alone 89.8 (n = 202/225) 78.4 (n = 40/51) 64.6 (n = 31/48)
Combination testingb 80.9 (n = 182/225) 60 (n = 30/50)c 33.3 (n = 16/48)
Specificity % (n = negative/total) RT-qPCR alone 92.6 (n = 239/258) 97.9 (n = 46/47) 80 (n = 28/35)
Combination testingb 94.6 (n = 244/258) 97.9 (n = 46/47) 100 (n = 35/35)
Accuracy % (n = true result/total) RT-qPCR alone 91.3 (n = 441/483) 87.8 (n = 86/98) 71.1 (n = 59/83)
Combination testingb 88.2 (n = 426/483) 78.4 (n = 76/97) 61.4 (n = 41/83)
  1. The reference-standard for diagnosis of FIP was considered identification of FCoV antigen by immunohistochemistry in at least one tissue in association with appropriate histopathological changes, and cats were considered “without FIP” where FIP was excluded as a diagnosis.
  2. aAs some positive samples without histopathological data were not subjected to sequencing, only those samples with histopathological data available were included in these calculations.
  3. bRT-qPCR in combination with spike protein sequence characterisation FCoV.
  4. cOne sample lost from analysis.