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Table 2 Origin of samples, RT-qPCR results and threshold cycle values

From: Limitations of using feline coronavirus spike protein gene mutations to diagnose feline infectious peritonitis

Sample source Number of samples analysed by RT-qPCR RT-qPCR positive result (%) Threshold cycle for positive samples—median; range (relative copy number—median; range)
Cats with FIP
 Tissue 260 235 (90.4) 26.2; 12.4 to 39.7 (1.1 × 104; 1.2 × 108 to 1.2)
 Fluid 51 40 (78.4) 31.2; 19.4 to 38.5 (373; 1.1 × 106 to 2.7)
  Body cavity fluid 35 32 (91.4) 30.9; 19.4 to 38.5 (457; 1.2 × 108 to 1.2)
   Abdominal 23 21 (91.3) 30.8; 22.2 to 38.5 (488; 1.6 × 105 to 2.7)
   Pleural 9 9 (100.0) 29.6; 19.4 to 35.0 (1.1 × 10−3 1.2 × 108 to 29)
   Pericardial 2 1 (50.0) 38.5 (2.7)
   Unrecorded 1 1 (100.0) 37.0 (7.5)
  CSF 14a 7 (50.0) 34.8; 27.9 to 36.5 (33; 3.4 × 103 to 11)
  Aqueous humour 2 1 (50.0) 33.7 (6.9)
 Faeces 48 31 (64.6) 30.9; 15.8 to 39.7 (457; 1.2 × 107 to 1.2)
  Whole faeces 42 28 (66.7) 31.0; 15.8 to 37.7 (427; 1.2 × 107 to 4.7)
  Faecal swab 6 3 (50.0) 30.5; 30.5 to 39.7 (598; 598 to 1.2)
Cats without FIP
 Tissue 258 20 (7.8) 36.2; 26.4 to 38.5 (12.9; 9.4 × 103 to 2.7)
 Fluid 47 1 (2.1) 36.4 (11)
  Body cavity 28 1 (3.6) 36.4 (11)
   Abdominal 13 1 (7.8) 36.4 (11)
   Pleural 12 0  
   Pericardial 3 0  
  CSF 19 0  
 Faeces 35 7 (20.0)  
  Whole faeces 33 7 (20.0) 33.6; 25.7 to 38.6 (74; 1.5 × 104 to 2.6)
  Faecal swab 2 0 33.6; 25.7 to 38.6 (74; 1.5 × 104 to 2.6)
  1. aAll but one sample was obtained from the cerebellomedullary cistern, one sample was collected from the lumbar subarachnoid space.