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Table 1 Primer and probe sequences used in this study

From: Limitations of using feline coronavirus spike protein gene mutations to diagnose feline infectious peritonitis

Name

Use

Sequence (5′–3′)

P009

qPCR forward primer

AGCAACTACTGCCACRGGAT

P010

qPCR reverse primer

GGAAGGTTCATCTCCCCAGT

TaqMan-P1

FCoV qPCR fluorescent probe

FAM-AATGGCCACACAGGGACAACGC-BHQ1

F614

Forward pyrosequencing primer

GCHCARTATTAYAATGGCATAATGG

R766

Biotinylated reverse pyrosequencing primer

BIO-AAGYCTRGCYTGYACTTGCAT

S680

M1058L pyrosequencing primer

ACAGCCTCDTTAATAGGVGGTATG

S693A

S1060A pyrosequencing primer

TAGGRGGTATGGCYWTGG

FCoV S2 F1

Forward FCoV type 2 spike gene fragment amplification primer

TCTGCTGCCATCAAAATCAC

FCoV S2 R3

Reverse FCoV type 2 spike gene fragment amplification primer

CGATGTGTAAGCAATTGTCCA

  1. qPCR: quantitative polymerase chain reaction, FCoV: feline coronavirus, FAM: fluorescein amidite, BHQ1: black hole quencher-1, BIO: biotin.
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Veterinary Research

ISSN: 1297-9716