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Figure 1 | Veterinary Research

Figure 1

From: Oral vaccination of wildlife using a vaccinia–rabies-glycoprotein recombinant virus vaccine (RABORAL V-RG®): a global review

Figure 1

Construction of the vaccinia–rabies glycoprotein recombinant vaccine (V-RG). The SAD (Street Alabama Dufferin; Wandeler, 1991) rabies virus strain (a) was isolated from the salivary glands of a rabid dog in Alabama (USA) during 1935 and was attenuated to the ERA (Evelyn Rokitnicki Abelseth, 1964 [23]) rabies virus strain by repeated cell culture passages (b). The complementary DNA corresponding to the gene coding for the 524 amino acid G protein of rabies virus strain ERA was inserted into the double-stranded DNA genome of the vaccinia virus strain Copenhagen, under the control of (c) the 7.5 kDa vaccinia virus protein promoter [143], in the gene coding for thymidine kinase (TK). Shown is the TK region of the vaccinia virus genome with the inserted rabies virus G-cDNA from HindIII-digested plasmid pTG187-PRO.

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