Neutralizing immune responses induced by oligomeric H5N1-hemagglutinins from plants

Table 1 Expression and functionality profiles of recombinant influenza hemagglutinin and S·Protein variants

Proteins	Protein expression confirmed by Western blot	Hemagglutination unit (HAU)
Proteins	Protein expression confirmed by Western blot	Plant extracts containing each single recombinant protein	Combination with H5-S·Tag in vitro
H5-S·Tag	(+)	0
S·Protein	(+)	0	0
S·Protein-pII	(+)	0	0
S·Protein-GCN4	(+)	0	0
S·Protein-TP	(+)	0	0

Each single protein was transiently expressed in plants. The expression of proteins was confirmed by Western blot. Plant extracts containing S·Protein variants were combined with the H5-S·Tag containing plant extract in vitro. Mixtures were rotated at 4 °C for 1 h. The oligomer formation of all variants was investigated by hemagglutination assay. (+): expression of a single protein confirmed by Western blot.

ISSN: 1297-9716