ID | Indel | Amino acid mutation | GFP expression (%) |
---|---|---|---|
WT | WT | No mutation | Strong (92.6) |
#16 | −12 bp | 4 amino acid deletion (76th to 79th positions) | Strong (91.8) |
#13 | −15 bp | 5 amino acid deletion (77th to 80th positions) | Moderate (53.3) |
#28 | −15 bp | 5 amino acid deletion (77th to 80th positions) | Moderate (49.2) |
#19 | −26 bp | Premature stop codon | Low (12.8) |
#17 | +44Â bp | Premature stop codon | Low (8.3) |
#27 | −17 bp | Premature stop codon | Low (6.2) |
#2 | −11 bp | Premature stop codon | Low (9.3) |
#10 | −8 bp | Premature stop codon | Low (5.9) |
#14 | −8 bp | Premature stop codon | Low (10.9) |
#18 | −8 bp | Premature stop codon | Low (13.1) |
#21 | −7 bp | Premature stop codon | Low (6.5) |
#23 | −7 bp | Premature stop codon | Low (6.5) |
#25 | −7 bp | Premature stop codon | Low (6.7) |