Genetic modification of
by CRISPR/Cas9 in DF-1 cells. A Gene structure of tvb (TNFRSF10B) and recognition sites of TVB#1 and TVB#2 CRISPR/Cas9 vectors. Blue bars indicate guide RNA recognition sites, and red bars indicate protospacer-adjacent motif (PAM) sequences. Scale bar = 1 kb. B T7E1 assay for DF-1 cells transfected with TVB#1 and TVB#2 CRISPR/Cas9 vectors. Bands cleaved by T7E1 endonuclease were seen in the experimental groups. C Sequencing analysis of transfected DF-1 cells using the TA cloning method. Grey letters indicate insertions, and grey letters with lines indicate deletions. Indel mutations and their frequencies are presented. Blue bars indicate guide RNA recognition sites, and red bars indicate PAM sequences. Wild type (WT) DF-1 cells were used as the control.