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Table 1 Passive protection test results of A/J mice.

From: Use of the mice passive protection test to evaluate the humoral response in goats vaccinated with Sterne 34F2 live spore vaccine

Goat serum

Survival (Time to deathb)

Animal

PA-ELISA (week 0)

TNA (week 0)

Undiluted sera

1:5

1:10

1:50

1:100

1:1000

D5a

2680 (212)

3410 (0)

3/3

3/3

2/3 (11)

0/3 (4, 4, 4)

1/3 (3, 3)

0/3 (2, 2, 2)

D6a

4190 (380)

1800 (0)

2/3 (12)

3/3

2/3 (5)

0/3 (3, 3, 3)

0/3 (2, 3, 3)

1/3 (2, 3)

D20a

8050 (310)

2530 (0)

2/3 (14)

3/3

1/3 (4, 7)

1/3 (3, 5)

0/3 (3, 3, 12)

0/3 (2, 2, 3)

D29a

7510 (240)

2280 (0)

3/3

2/3 (5)

2/3 (10)

1/3 (3, 5)

1/3 (3, 8)

1/3 (3, 3)

D31a

1450 (220)

240 (0)

3/3

1/3 (4, 7)

0/3 (3, 3, 4)

0/3 (3, 3, 4)

0/3 (3, 3, 4)

1/3 (2, 3)

Negative controlac

 D13

590 (80)

0 (0)

0/3 (2, 2, 2)

     

 D39

100 (130)

0 (0)

0/3 (3, 2, 3)

     

 D77

120 (90)

0 (0)

0/3 (3, 3, 2)

     

Positive controld

 8175

39 590 (212)

5870 (0)

3/3

     

 8182

17 000 (0)

7300 (0)

3/3

     

 8210

185 500 (173)

17 380 (0)

3/3

     

 8212

1 223 430 (405)

35 720 (0)

3/3

     
  1. aSera collected at 17 weeks
  2. bTime to death (in days) of non-surviving mice; Mice injected with undiluted and diluted hyper-immune goat sera and challenged with spores from the Sterne 34F2 vaccine strain 24 h later and immune sera were diluted from 1:5 to 1:1000
  3. cSera collected from goats (n = 3) receiving sterile saline only; all mice died within 3 days of challenge with B. anthracis Sterne strain
  4. dSera sourced from goats (n = 4) surviving virulent anthrax spores after two vaccinations with the Sterne live spore vaccine in a previous study [12]
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Veterinary Research

ISSN: 1297-9716