|
Goat serum
|
Survival (Time to deathb)
|
|---|
|
Animal
|
PA-ELISA (week 0)
|
TNA (week 0)
|
Undiluted sera
|
1:5
|
1:10
|
1:50
|
1:100
|
1:1000
|
|---|
|
D5a
|
2680 (212)
|
3410 (0)
|
3/3
|
3/3
|
2/3 (11)
|
0/3 (4, 4, 4)
|
1/3 (3, 3)
|
0/3 (2, 2, 2)
|
|
D6a
|
4190 (380)
|
1800 (0)
|
2/3 (12)
|
3/3
|
2/3 (5)
|
0/3 (3, 3, 3)
|
0/3 (2, 3, 3)
|
1/3 (2, 3)
|
|
D20a
|
8050 (310)
|
2530 (0)
|
2/3 (14)
|
3/3
|
1/3 (4, 7)
|
1/3 (3, 5)
|
0/3 (3, 3, 12)
|
0/3 (2, 2, 3)
|
|
D29a
|
7510 (240)
|
2280 (0)
|
3/3
|
2/3 (5)
|
2/3 (10)
|
1/3 (3, 5)
|
1/3 (3, 8)
|
1/3 (3, 3)
|
|
D31a
|
1450 (220)
|
240 (0)
|
3/3
|
1/3 (4, 7)
|
0/3 (3, 3, 4)
|
0/3 (3, 3, 4)
|
0/3 (3, 3, 4)
|
1/3 (2, 3)
|
|
Negative controlac
|
|
D13
|
590 (80)
|
0 (0)
|
0/3 (2, 2, 2)
| | | | | |
|
D39
|
100 (130)
|
0 (0)
|
0/3 (3, 2, 3)
| | | | | |
|
D77
|
120 (90)
|
0 (0)
|
0/3 (3, 3, 2)
| | | | | |
|
Positive controld
|
|
8175
|
39 590 (212)
|
5870 (0)
|
3/3
| | | | | |
|
8182
|
17 000 (0)
|
7300 (0)
|
3/3
| | | | | |
|
8210
|
185 500 (173)
|
17 380 (0)
|
3/3
| | | | | |
|
8212
|
1 223 430 (405)
|
35 720 (0)
|
3/3
| | | | | |
-
aSera collected at 17 weeks
-
bTime to death (in days) of non-surviving mice; Mice injected with undiluted and diluted hyper-immune goat sera and challenged with spores from the Sterne 34F2 vaccine strain 24 h later and immune sera were diluted from 1:5 to 1:1000
-
cSera collected from goats (n = 3) receiving sterile saline only; all mice died within 3 days of challenge with B. anthracis Sterne strain
-
dSera sourced from goats (n = 4) surviving virulent anthrax spores after two vaccinations with the Sterne live spore vaccine in a previous study [12]
