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Table 1 Passive protection test results of A/J mice.

From: Use of the mice passive protection test to evaluate the humoral response in goats vaccinated with Sterne 34F2 live spore vaccine

Goat serum Survival (Time to deathb)
Animal PA-ELISA (week 0) TNA (week 0) Undiluted sera 1:5 1:10 1:50 1:100 1:1000
D5a 2680 (212) 3410 (0) 3/3 3/3 2/3 (11) 0/3 (4, 4, 4) 1/3 (3, 3) 0/3 (2, 2, 2)
D6a 4190 (380) 1800 (0) 2/3 (12) 3/3 2/3 (5) 0/3 (3, 3, 3) 0/3 (2, 3, 3) 1/3 (2, 3)
D20a 8050 (310) 2530 (0) 2/3 (14) 3/3 1/3 (4, 7) 1/3 (3, 5) 0/3 (3, 3, 12) 0/3 (2, 2, 3)
D29a 7510 (240) 2280 (0) 3/3 2/3 (5) 2/3 (10) 1/3 (3, 5) 1/3 (3, 8) 1/3 (3, 3)
D31a 1450 (220) 240 (0) 3/3 1/3 (4, 7) 0/3 (3, 3, 4) 0/3 (3, 3, 4) 0/3 (3, 3, 4) 1/3 (2, 3)
Negative controlac
 D13 590 (80) 0 (0) 0/3 (2, 2, 2)      
 D39 100 (130) 0 (0) 0/3 (3, 2, 3)      
 D77 120 (90) 0 (0) 0/3 (3, 3, 2)      
Positive controld
 8175 39 590 (212) 5870 (0) 3/3      
 8182 17 000 (0) 7300 (0) 3/3      
 8210 185 500 (173) 17 380 (0) 3/3      
 8212 1 223 430 (405) 35 720 (0) 3/3      
  1. aSera collected at 17 weeks
  2. bTime to death (in days) of non-surviving mice; Mice injected with undiluted and diluted hyper-immune goat sera and challenged with spores from the Sterne 34F2 vaccine strain 24 h later and immune sera were diluted from 1:5 to 1:1000
  3. cSera collected from goats (n = 3) receiving sterile saline only; all mice died within 3 days of challenge with B. anthracis Sterne strain
  4. dSera sourced from goats (n = 4) surviving virulent anthrax spores after two vaccinations with the Sterne live spore vaccine in a previous study [12]