The reduction of ROS levels by PLC inhibitor U73122 in MDBK cells following BoHV-1 infection. MDBK cells subjected to a pretreatment with DMSO, U73122 (2.5 μM) (A) or edelfosine (5 μM) (B) for 1 h, were mock infected with supernatant of cell culture or infected with BoHV-1 along with chemical inhibitors. At 4 hpi cellular ROS were detected using H2DCFDA (5 μM, 30 min), and the quantification of fluorescence intensity was analyzed using software Image-pro Plus 6. Values represent three independent experiments. Significance was assessed with the student t test (*P < 0.05).