Figure 3

BoHV-1 infection enhanced the phosphorylation of PLCγ-1 (S1248) in MDBK cells. (A) Time course of PLCγ-1(Ser1248) phosphorylation following BoHV-1 infection. Serum starved MDBK cells were infected with BoHV-1 at an MOI of 10, and at indicated time points the cell lysates were prepared for western blotting. (B) MDBK cells were incubated with UV-irradiation inactivated virus or infectious virus at an MOI of 10 for 30 min, and then subjected to analysis of PLCγ-1(Ser1248) phosphorylation by western blotting. (C) The ability of UV-irradiation inactivated virus to bind to MDBK cells were analyzed with FACS. MDBK cells with or without viral particles attached were stained with bovine anti-BoHV-1 serum followed by FITC-conjugated anti-bovine IgG. The cell membrane bound viral particles were analyzed on FACS. The mean fluorescence intensity was analyzed. (D) Serum starved MDBK cells were infected with BoHV-1 at an MOI of 10 along with PAA treatment (100 μM). At 0.5 and 12 hpi the cell lysates for western blotting analysis were prepared, respectively. At 24 hpi the virus yield were determined in MDBK cells (E). The band intensity was analyzed with software image J. Data are representative results of three independent experiments.