Both U73122 and edelfosine affect virus entry. Serum starved MDBK cells infected with BoHV-1 (MOI = 1) were treated with U73122 (A) and edelfosine (B) at a concentration of 5 μM, at the virus entry, or post-entry process, respectively. At 24 hpi the viral titer were determined in MDBK cells with results expressed as TCID50/mL. C Serum starved MDBK cells were incubated in the absence or presence of 5 μM of U73122 or 5 μM of edelfosine for 1 h at 37 °C. The virus in ice-cold DMEM containing fresh inhibitors was then allowed to adsorb to MDBK cells for 1 h at 4 °C. After freezing and thawing, the cell membrane bound viruses were titered in MDBK cells. Values represent three independent experiments. Significance was assessed with the student t test (*P < 0.05).