Infection characteristics of the isolated PEDV strain 85-7. (A) A distinct CPE (P5) was observed and photographed at 12, 24, 36, 48 hpi (magnification of 100×). (B) For IFA assay of P5, cells were fixed and incubated with PEDV-specific monoclonal antibody 5E2-C4, followed by Cy3-labeled Goat Anti-Mouse IgG (H+L) (secondary antibody), and then examined by fluorescence microscopy (magnification of 100×). (C) A thin section of infected cells (P5) at 24 hpi showing accumulation of virus particles in the cytoplasm membrane and cytoplasm (magnification 1200× and 2500×, Bar = 200 nm. Some virus particles are indicated by red arrows. An enlarged image of part of the cytoplasm is shown.