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Table 4 Gene expression in five organs of rainbow trout fry at 4 h, day 2 and day 7 post-initial vaccination

From: Efficacy of a polyvalent immersion vaccine against Flavobacterium psychrophilum and evaluation of immune response to vaccination in rainbow trout fry (Onchorynchus mykiss L.)

 

4 h

Day 2

Day 7

Gill

 TLR-2

1.835 ± 0.48

2.427 ± 0.73

1.776 ± 0.73

 C3

0.878 ± 0.61

1.06 ± 0.76

1.215 ± 0.66

 IL-1β

0.654 ± 0.41

0.512 ↓ ± 0.34

0.651 ± 0.43

 IgT

1.041 ± 0.64

1.096 ± 0.72

0.713 ± 0.50

 IgM

0.713 ± 0.34

1.344 ± 0.85

1.088 ± 0.66

 CD4

0.914 ± 0.66

0.975 ± 0.83

0.922 ± 0.70

 CD8

0.81 ± 0.58

0.76 ± 0.38

1.071 ± 0.58

Hind-gut

 TLR-2

1.808 ± 0.55

1.5 ± 0.86

0.562 ± 0.30

 C3

0.444 ± 0.09

0.485 ± 0.163

0.611 ± 0.15

 IL-1β

1 ± 0.51

1.018 ± 0.54

0.678 ± 0.37

 IgT

0.742 ± 0.32

2.050 ↑ ± 1.23

0.23 ± 0.02

 IgM

1.381 ± 0.72

2.035 ↑ ± 1.16

0.51 ± 0.15

 CD4

1.126 ± 0.93

1.476 ± 1.01

0.853 ± 0.46

 CD8

0.873 ± 0.54

0.748 ± 0.37

0.538 ± 0.25

Skin

 TLR-2

2.08 ± 0.89

4.348 ↑ ± 1.82

1.611 ± 0.53

 C3

1.038 ± 0.73

0.958 ± 0.83

1.093 ± 0.85

 IL-1β

1.019 ± 0.59

0.475 ± 0.23

0.971 ± 0.54

 IgT

1.183 ± 0.75

1.322 ± 0.54

0.491 ± 0.24

 IgM

0.73 ± 0.53

1.729 ± 0.77

1.1 ± 0.34

 CD4

0.772 ± 0.46

1.272 ± 0.88

0.974 ± 0.71

 CD8

0.679 ± 0.34

0.711 ± 0.31

0.713 ± 0.42

Head kidney

 TLR-2

1.031 ± 0.45

0.918 ± 0.56

0.791 ± 0.50

 C3

0.677 ± 0.10

1.444 ± 0.43

5.26 ± 0.67

 IL-1β

1.819 ↑ ± 0.90

1.001 ± 0.42

1.347 ± 0.64

 IgT

0.995 ± 0.51

1.199 ± 0.85

2.189 ± 0.20

 IgM

0.988 ± 0.71

0.784 ± 0.53

0.654 ± 0.42

 CD4

0.944 ± 0.66

1.128 ± 0.83

1.191 ± 0.83

 CD8

0.788 ± 0.56

0.426 ↓ ± 0.21

0.947 ± 0.47

Spleen

 TLR-2

1.823 ± 0.54

2.017 ± 0.687

1.037 ± 0.37

 C3

0.793 ± 0.39

1.205 ± 0.63

0.67 ± 0.32

 IL-1β

2.494 ± 0.98

0.734 ± 0.44

1.08 ± 0.75

 IgT

0.898 ± 0.41

1.572 ± 0.737

1.23 ± 0.46

 IgM

1.184 ± 0.60

0.734 ± 0.34

0.649 ± 0.41

 CD4

0.869 ± 0.63

0.935 ± 0.61

0.782 ↓↓ ± 0.61

 CD8

0.701 ± 0.46

0.469 ± 0.24

0.554 ↓ ± 0.36

  1. Fold change of genes in vaccinated groups compared to controls ± SE.
  2. ↑ and ↓ indicate significant up-regulation and down-regulation relative to control respectively (p < 0.05). ↓↓ indicate significant up-regulation and down-regulation relative to control respectively (p < 0.01).