Table 1 Details of primers used in RT- qPCR
Gene | Oligo sequence | Product size (bp) | Annealing temperature (°C) | Genbank accession no. |
|---|---|---|---|---|
EF-1α | F: GATCCAGAAGGAGGTCACCA R: TTACGTTCGACCTTCCATCC | 150 | 58 | NM_001124339.1 |
β-actin | F: CAGCCCTCCTTCCTCGGTAT R: AGCACCGTGTTGGCGTACA | 110 | 54 | NM_001124235.1 |
TLR-2 | F: GATCCAGAGCAACACTCTCAACAT R: CTCCAGACCATGAAGTTGACAAAC | 282 | 58 | LK933545.1 |
C3 | F: GAGATGGCCTCCAAGAAGATAGAA R: ACCGCATGTACGCATCATCA | 91 | 58 | L24433.1 |
IL1β | F: GACATGGTGCGTTTCCTTTT R: ACCGGTTTGGTGTAGTCCTG | 122 | 54 | AJ278242.2 |
IgT | F: AACATCACCTGGCACATCAA R: TTCAGGTTGCCCTTTGATTC | 80 | 54 | AY870266.1 |
IgM | F: TGCGTGTTTGAGAACAAAGC R: GACGGCTCGATGATCGTAAT | 107 | 54 | AH014877.2 |
CD4-1 | F: GAGTACACCTGCGCTGTGGAAT R: GGTTGACCTCCTGACCTACAAAGG | 121 | 58 | NM_001124539.1 |
CD8a | F: ACGACTACACCAATGACCACAACC R: CAGTGATGATGAGGAGGAGGAAGA | 160 | 58 | AF178055.1 |