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Table 1 List of plasmid, bacterial and viral strains used

From: Intracellular delivery of HA1 subunit antigen through attenuated Salmonella Gallinarum act as a bivalent vaccine against fowl typhoid and low pathogenic H5N3 virus

Strains/plasmid

Description

Reference

S. Gallinarum

 JOL967

∆lon, ∆cpxR, ∆asd mutant of S. Gallinarum

[16]

 JOL394

Wild-type S. Gallinarum from chicken with fowl typhoid

 

 JOL-1814

JOL967 with pMMP65-HA1 plasmid

This study

 JOL1820

JOL967 with pMMP65 plasmid

This study

 BL21

E. coli cells for protein expression

 

H5N3 virus

Influenza A/spot-billed duck/Korea/KNU SYG06/2006 LPAI

 

Plasmids

 pMMP65

Asd+, pBR ori, -lactamase signal sequence-based periplasmic secretion plasmid, 6 His tag

 

 pMMP65-HA1

pMMP65 harboring HA1 gene of H5N3 virus

This study

 pET28a(+) HA1

pET28(+) harborunf HA1 gene of H5N3 virus

 
  1. The strains were maintained as frozen glycerol cultures in LB broth at −70 °C.