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Figure 2 | Veterinary Research

Figure 2

From: Intracellular delivery of HA1 subunit antigen through attenuated Salmonella Gallinarum act as a bivalent vaccine against fowl typhoid and low pathogenic H5N3 virus

Figure 2

In vitro delivery of HA1 antigen to peritoneal macrophages via JOL1814. Harvested and cultured peritoneal macrophages were infected with JOL1814 and JOL1820 at an MOI of 100. The cells were fixed at 24 hpi and analysed for immunofluorescence by fluorescence microscopy (total magnification, 10 × 100). The JOL1814 infected peritoneal macrophages showed green and red fluorescence surrounding the blue fluorescence nuclei. The vector control JOL1820 showed only green fluorescence surrounding the blue fluorescence nuclei. DAPI: blue coloured nuclear stain; Rhodamine: tagged antibodies for localization of the HA1 antigen and FITC: tagged antibodies for localization of S. Gallinarum.

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Veterinary Research

ISSN: 1297-9716