Skip to main content
Figure 1 | Veterinary Research

Figure 1

From: Intracellular delivery of HA1 subunit antigen through attenuated Salmonella Gallinarum act as a bivalent vaccine against fowl typhoid and low pathogenic H5N3 virus

Figure 1

Schematics for construction of the pMMP65-HA1 constitutive expression plasmid and Western blot analysis of JOL1814. A Cloning of the HA1 gene (amino acid residues 63–286) from influenza. A H5N3 virus into pMMP65 under a constitutive Ptrc promoter in frame with the bla periplasmic signal sequence. B Detection of HA1 protein expressed and secreted by JOL1814 with H5N1, a hemagglutinin (HA) (bird flu) polyclonal antibody. Lane M, size marker; VC: JOL1820 vector control; Pellet: a cellular pellet of JOL1814; PF: periplasmic fraction of JOL1814.

Back to article page