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Figure 3 | Veterinary Research

Figure 3

From: Role of transcription regulatory sequence in regulation of gene expression and replication of porcine reproductive and respiratory syndrome virus

Figure 3

Effect of the HP-PRRSV TRSs on EGFP expression in Marc-145 cells infected with rHP-PRRSVs. Marc-145 cells were infected at an MOI of 0.01 and cultured until 60% of cells showed the cytopathic effect (CPE). For western blot analysis of EGFP production, total proteins were collected from virus-infected cells, electrophoresed, transferred to a polyvinylidene difluoride (PVDF) membrane, and immunostained using a mouse anti-GFP monoclonal antibody, anti-α-Tubulin antibody and anti-PRRSV N protein as a loading control.

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