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Table 1 Strains and plasmids used in the study

From: Serotype-specific role of antigen I/II in the initial steps of the pathogenesis of the infection caused by Streptococcus suis

Strain or plasmid Characteristics References
Streptococcus suis
 SC84 Serotype 2 strain isolated from a patient with streptococcal toxic shock-like syndrome in China [47]
 1135776 Serotype 9 strain isolated from pig following sudden death in Canada This study
 S2ΔagI/II SC84-derived strain carrying an in-frame deletion of the agI/II gene This study
 S9ΔagI/II 1135776-derived strain carrying an in-frame deletion of the agI/II gene This study
 S2CΔagI/II SC84-derived strain carrying pOri23-S2agI/II This study
 S9CΔagI/II 1135776-derived strain carrying pOri23-S9agI/II This study
Escherichia coli
 TOP10 Host for pCR2.1 and pSET4s derivatives Invitrogen
 MC1061 Host for pOri23 derivatives [16]
 BL21(DE3) Host for pET151 derivatives Invitrogen
Plasmids
 pET151 Apr, pBR322 ori, T7 promotor Invitrogen
 pCR2.1 Apr, Kmr, pUC ori, lacZΔM15 Invitrogen
 pSET4s Spcr, pUC ori, thermosensitive pG+host3 ori, lacZΔM15 [37]
 pOri23 Ermr, ColE1 ori, P23 [28]
 pET151–S2agI/II pET151 carrying the S2 agI/II gene This study
 pSET4s–S2agI/II pSET4s carrying regions upstream and downstream of the S2 agI/II gene This study
 pSET4s–S9agI/II pSET4s carrying regions upstream and downstream of the S9 agI/II gene This study
 pOri23spc–S2agI/II pOri23 carrying the S2 agI/II gene as well as its promotor and terminator This study
 pOri23spc–S9agI/II pOri23 carrying the S9 agI/II gene as well as its promotor and terminator This study