Figure 4

Specific immune responses from the different breeds of sheep inoculated with MAP. A MAP specific antibody responses. Data shown are Geometric means of the MAP specific SP% from the assay for each breed over the trial. Error bars indicate the standard error. ×, Significant difference between the White Suffolk × Merino animals and the Border Leicester and Merino breeds (p < 0.05). *, significant differences between the White Suffolk × Merino and Merino breeds (p < 0.05). **, significant differences between the White Suffolk × Merino and the other breeds (p < 0.05). B MAP specific antibody responses from animals with clinical disease, quantified at the last sampling taken before or at necropsy. MAP specific antibody responses were measured by a commercial ELISA (Institut Porquier from IDEXX); the blue horizontal line at SP% 55 represents the positive–negative cut point for the assay. C MAP specific interferon gamma responses from the different breeds of experimentally inoculated sheep. Data shown are geometric means of the MAP specific SP% from the assay. Error bars indicate the standard error. Poll Dorset and Border Leicester animals had a significantly (p > 0.01) greater than the Merino and White Suffolk × Merino. ^#Poll Dorset animals had a significantly greater mean response to the other breeds (p > 0.0001), the White Suffolk had a significantly lower response that the Merino and Border Leicester (p > 0.05). ^§Poll Dorset animal’s response significantly greater response than the Merino and White Suffolk × Merino breeds (p > 0.005). **The Border Leicester breed had a significantly greater response than White Suffolk × Merino (p > 0.01). ^##At 12 months post inoculation the White Suffolk × Merino breed had a significantly lower response than the other breeds (p > 0.05).