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Table 3 Doses of bacteria (bacteria/mL) in contaminated seawater inducing more or less than 50% of mortality after 24 h of immersion of 10 oysters per condition in individuals beakers (oyster batch 2)

From: Several strains, one disease: experimental investigation of Vibrio aestuarianus infection parameters in the Pacific oyster, Crassostrea gigas

Strains

Dose inducing less than 50% mortality

Dose inducing more than 50% mortality

12/016

7.5 × 104 − 2.82 × 105

1.27 × 106

02/041

6.7 × 104 − 5.75 × 105

1.70 × 106

12/063

2.4 × 104 − 3.90 × 105

2.26 × 106

02/092

5.3 × 104 − 1.34 × 105

0.61 × 106

07/115

1.48 × 106

n.d.

  1. Different strains of V. aestuarianus (02/041-GFP, 02/041, 02/092, 07/115, 12/016, 12/063) were used to produce contaminated seawaters: source oysters were intramuscularly injected with 5 × 107 bacteria/animal. After 18 h, contaminated seawaters were serially diluted in fresh UV-treated seawater, and bacterial concentration was estimated by QPCR. Experimental design is described in Additional file 1.
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Veterinary Research

ISSN: 1297-9716