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Table 2 Effects of Matrix-M on gene expression (relative gene expression versus untreated control) in cultures of porcine monocytes, lymphocytes and monocyte-derived dendritic cells (MoDC)

From: Innate immune responses induced by the saponin adjuvant Matrix-M in specific pathogen free pigs

Target name

Lymphocytes 3 daysa

Monocytes 1 daya

MoDC 5 daysa

Matrix-M 6 hb

Matrix-M 3 daysb

Matrix-M 6 hb

Matrix-M 6 hb

Matrix-M 5 daysb

n = 8c

n = 8c

n = 10c

n = 6c

n = 6c

IL-1β

2.0

9.6*

3.3*

IL-6

< >

3.9

2.1

< >

10.2*

TNF-α

2.5

< >

< >

CXCL8

2.4*

5.9**

3.2**

< >

< >

IL-12p40

2.6*

2.6*

nd

IL-17A

0.3

27.8*

nd

IFN-γ

< >

4.8*

nd

IL-10

0.2*

0.4*

< >

TGF-β

< >

< >

IFN-α

< >

< >

< >

< >

3.8*

IFN-β

nd

nd

nd

< >

2.8

IFITM3

< >d

< >d

< >

< >d

< >d

SPP1

< >d

< >d

< >e

< >d

0.2d

STING

< >d

< >d

< >d

< >d

TLR2

0.4**

0.2

< >

0.3*

0.2*

  1. < >, gene not affected, i.e. 0.5 < FC < 2.
  2. nd not detected; too few samples showed any expression of the gene of interest to allow FC calculation, – not analysed.
  3. p < 0.05 and ** p < 0.01 for difference in expression between Matrix-M treated samples and medium control (i.e. FC ≠ 1); Wilcoxon matched-pairs signed rank test.
  4. aTotal culture time.
  5. bMatrix-M exposure time.
  6. cUnless otherwise indicated.
  7. d n = 4.
  8. e n = 6.