Figure 1
From: Innate immune responses induced by the saponin adjuvant Matrix-M in specific pathogen free pigs
Experimental design. Sixteen SPF pigs were treated (0 h) with Matrix M (n = 8) or saline (n = 8) the evening before a 2-h transport to the animal facility. At arrival (18 h), the SPF pigs were allotted to three rooms. SPF pigs given Matrix-M or saline were mixed in the first room (SPFMatrix-M/SPF and SPFSaline/SPF; n = 4 + 4). Four hours later (22 h) eight conventionally reared pigs (Conv) arrived and were mixed with four of the SPF pigs given Matrix-M (SPFMatrix-M/Conv) or four of those given saline (SPFSaline/Conv; n = 4) in the other two rooms. Blood samples were collected from the SPF pigs in tubes without additives (serum), EDTA tubes and PAXgene Blood RNA tubes as indicated. Clinical examination was performed concurrently with blood sampling and at least once more each day. Post-mortem examination was performed on all pigs at termination of the experiment (6 days).