Figure 2
Histological lesions and immunohistochemical detection of viral antigen in 2-week old Pekin ducks and Chinese geese inoculated by the intrachoanal route with H5N8 and H5N2 HPAI viruses. 40×; viral antigen staining in red. A Heart. Goose inoculated with H5N2, 8 dpi; lymphoplasmacytic inflammation. B Skeletal muscle. Goose inoculated with H5N2, 10 dpi; lymphoplasmacytic inflammation. C Cerebrum. Goose inoculated with H5N2, 8 dpi; foci of malacia, and gliosis. D Cerebrum. Goose inoculated with H5N2, 10 dpi; viral antigen in neurons and glial cells. E Spleen. Goose inoculated with H5N2, 8 dpi; viral antigen in necrotic cells and mononuclear cells. F Airsac. Pekin duck inoculated with H5N8, 4 dpi; viral antigen present in epithelial cells. G Harderian gland. Pekin duck inoculated with H5N8, 4 dpi; viral antigen present in epithelial cells and infiltrating monocytes. H Lung. Pekin duck inoculated with H5N8, 4 dpi; viral antigen in epithelium of air capillaries and infiltrating monocytes.