Identification of recombinant baculoviruses displaying complement interfering factors. A Schematic diagram of donor vectors of recombinant baculoviruses displaying complement interfering factors on the surface. CMV: human cytomegalovirus (CMV) immediate early enhancer and promoter, ph: AcMNPV polyhedrin promoter, p10: AcMNPV p10 promoter, gp64 SP: Autographa californica nuclear polyhedrosis virus (AcMNPV) gp64 signal sequence, MCS: multiple cloning sites, gp64 TM: AcMNPV gp64 transmembrane domain, gp64 CTD: AcMNPV gp64 cytoplasmic domain, VSVG TM: vesicular stomatitis virus G protein (VSV-G) transmembrane domain, VSVG CTD: VSV-G cytoplasmic domain. B Confocal microscopy analysis of His6-tagged pFc, SPICE, DAF and OmCI proteins anchoring on the plasma membrane of Sf-9 cells. Protein localization was visualized in cell membrane using a confocal microscope by the primary antibody (anti-His6 monoclonal antibodies) and the secondary antibody (FITC-conjugated goat anti-mouse IgG). C Western blot analysis of His6-tagged pFc (27 kDa), SPICE (26 kDa), DAF (36 kDa) and OmCI (18 kDa) in infected Sf-9 cells.