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Figure 5 | Veterinary Research

Figure 5

From: Concurrent infection with porcine reproductive and respiratory syndrome virus and Haemophilus parasuis in two types of porcine macrophages: apoptosis, production of ROS and formation of multinucleated giant cells

Figure 5

Expression rates of mRNA of selected apoptosis-related genes. Selected anti-apoptotic Bcl-2 (A, E) and pro-apoptotic p53 (B, F), Bad (C, G) and Bax (D, H) genes were measured by real-time PCR using gene specific primers at 4/28 and 24/48 h PI in MDMs (A, B, C, D) or PAMs (E, F, G, H). Boxplots indicate the median (middle line), 25th and 75th percentiles (boxes), and maximum and minimum (whiskers) of five independent experiments performed in culture duplicates. The degree of gene expression is displayed as fold change of the housekeeping gene hypoxanthine phosphoribosyltransferase (HPRT) expression. Mock-infected MDMs/PAMs served as controls for PRRSV infection. Significant differences between PRRSV and H. parasuis co-infected cells and other tested groups are denoted by different letters (a, b, c, d) (p < 0.05).

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Veterinary Research

ISSN: 1297-9716