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Table 1 Cell lines used in this study

From: Permissiveness of bovine epithelial cells from lung, intestine, placenta and udder for infection with Coxiella burnetii

Code Clone Origin Culture medium Source Reference
Organ Donor
L BEL-26 Lung Fetal Dulbecco’s Modified Eagle Medium (DMEM, 1.0 g/mL glucose) CCLVa (–)
G FKD-R 971 Jejunum Fetal Iscove’s Modified Dulbecco’s Medium (IMDM)/Ham’s F12 nutrient mix [1:1] CCLVa (–)
Pm BCEC Maternal placenta Mature/pregnant DMEM (4.5 g/mL glucose)/Ham’s F12 nutrient mix [1:1] TiHob [35]
Pf F3 Fetal placenta Mature/pregnant DMEM (4.5 g/mL glucose)/Ham’s F12 nutrient mix [1:1] TiHob [36]
U PS Udder Mature Advanced DMEM/Ham’s F12 nutrient mix [1:1] + additional componentsd INRAc [17]
  1. aCollection of Cell Lines in Veterinary Medicine (CCLV) at Friedrich-Loeffler-Institut (Isle of Riems), Germany; cell line FKD-R 971 was generated by Roland Riebe (RIE 971).
  2. bChristiane Pfarrer, Department of Anatomy, University of Veterinary Medicine, Hannover, Germany.
  3. cPascal Rainard, ISP, INRA, Université Tours, Nouzilly, France.
  4. dSee “Materials and methods”.