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Table 3 Commercial antibodies used in the detection of native intracellular IL-17A and IFN-γ by bovine and ovine T cell subsets

From: Enhancing the toolbox to study IL-17A in cattle and sheep

Antibody clone Commercial supplier and product code in brackets Antibody isotype Target antigen Host specificity Antibody conjugation Antibody dilution
CC8 Bio-Rad Laboratories (MCA1653PE) IgG2a CD4 Bovine Phycoerythrin 1:20
44.38 Bio-Rad Laboratories (MCA2213PE) IgG2a CD4 Ovine Phycoerythrin 1:20
CC58 Bio-Rad Laboratories (MCA1654PE) IgG1 CD8β Bovine Phycoerythrin 1:20
CC15 Bio-Rad Laboratories (MCA838PE) IgG2a WC-1 Bovine Phycoerythrin 1:200
CC302 Bio-Rad Laboratories (MCA1783A647) IgG1 IFN-γ Bovine Alexafluor-647 1:200
eBio64DEC17 eBiosciences (17-7179) IgG1 IL-17A Human APC 1:20
F8-11-13 Bio-Rad Laboratories (MCA1209PE) IgG1 Recognised rat cell surface marker Rat Phycoerythrin 1:20
MRC OX-34 Bio-Rad Laboratories (MCA929PE) IgG2a Rat cell surface marker Rat Phycoerythrin 1:20
  1. All details of antibody clones, product code, immunogen, host specificity and antibody conjugate are taken from datasheets provided by the commercial suppliers. The antibodies were used at the described dilutions for the cell phenotyping and intracellular cytokine staining described in “Expression of intracellular IL-17A and IFN-γ by bovine and ovine T cell subsets section”.