Table 3 Commercial antibodies used in the detection of native intracellular IL-17A and IFN-γ by bovine and ovine T cell subsets
From: Enhancing the toolbox to study IL-17A in cattle and sheep
Antibody clone | Commercial supplier and product code in brackets | Antibody isotype | Target antigen | Host specificity | Antibody conjugation | Antibody dilution |
|---|---|---|---|---|---|---|
CC8 | Bio-Rad Laboratories (MCA1653PE) | IgG2a | CD4 | Bovine | Phycoerythrin | 1:20 |
44.38 | Bio-Rad Laboratories (MCA2213PE) | IgG2a | CD4 | Ovine | Phycoerythrin | 1:20 |
CC58 | Bio-Rad Laboratories (MCA1654PE) | IgG1 | CD8β | Bovine | Phycoerythrin | 1:20 |
CC15 | Bio-Rad Laboratories (MCA838PE) | IgG2a | WC-1 | Bovine | Phycoerythrin | 1:200 |
CC302 | Bio-Rad Laboratories (MCA1783A647) | IgG1 | IFN-γ | Bovine | Alexafluor-647 | 1:200 |
eBio64DEC17 | eBiosciences (17-7179) | IgG1 | IL-17A | Human | APC | 1:20 |
F8-11-13 | Bio-Rad Laboratories (MCA1209PE) | IgG1 | Recognised rat cell surface marker | Rat | Phycoerythrin | 1:20 |
MRC OX-34 | Bio-Rad Laboratories (MCA929PE) | IgG2a | Rat cell surface marker | Rat | Phycoerythrin | 1:20 |