Skip to main content

Table 1 Commercial anti-IL-17A antibodies evaluated by intracellular staining for capacity to bind recombinant bovine and ovine IL-17A

From: Enhancing the toolbox to study IL-17A in cattle and sheep

Primary antibody used in the intracellular staining (CHO cells) Clone Commercial supplier and product code in brackets Antibody isotype Immunogen Specificity Antibody conjugation if present
A.1 N/A rabbit derived polyclonal antibody. Lot number BO1431JK Kingfisher Biotech (PB0274B-100) IgG1 Recombinant bovine IL-17A Bovine None
B.1 eBio64Dec17 eBiosciences (12-7179-41) IgG1 Recombinant human IL-17A Human PE
C.1 MT44.6 Mabtech (3520-3-250) IgG1 Recombinant human IL-17A Human, rhesus and cynomolgus macaques and common marmoset monkeys None
C.2 MT241 Mabtech (3520M-3-250) IgG1 Recombinant human IL-17A Human, rhesus and cynomolgus macaques None
C.3 MT2770 Mabtech (3521-14-250) IgG1 Recombinant mouse IL-17A Mouse None
C.4 MT504 Mabtech (3520-6-250) IgG1 Recombinant human IL-17A Human, rhesus and cynomolgus macaques and common marmoset monkeys Biotin
D.1 41809 R & D Systems IC317P IgG2b Recombinant human IL-17A amino acids 20-155 Human PE
D.2 41802 R & D Systems (IC3171P) IgG1 Recombinant human IL-17A amino acids 20-155 Human PE
  1. All details of antibody clone, product code, immunogen, host specificity and antibody conjugate have been taken from supplier datasheets. Each antibody has been assigned a code where the capital letter denotes the commercial supplier and the integer an individual pab or a mab clone. The commercial antibodies were used in conjunction with an appropriate control antibody (see Table 2, denoted by the equivalent letter but in lowercase) for the intracellular staining protocol described in “Evaluation of commercial antibodies section”.