Enhancing the toolbox to study IL-17A in cattle and sheep

Table 1 Commercial anti-IL-17A antibodies evaluated by intracellular staining for capacity to bind recombinant bovine and ovine IL-17A

Primary antibody used in the intracellular staining (CHO cells)	Clone	Commercial supplier and product code in brackets	Antibody isotype	Immunogen	Specificity	Antibody conjugation if present
A.1	N/A rabbit derived polyclonal antibody. Lot number BO1431JK	Kingfisher Biotech (PB0274B-100)	IgG1	Recombinant bovine IL-17A	Bovine	None
B.1	eBio64Dec17	eBiosciences (12-7179-41)	IgG1	Recombinant human IL-17A	Human	PE
C.1	MT44.6	Mabtech (3520-3-250)	IgG1	Recombinant human IL-17A	Human, rhesus and cynomolgus macaques and common marmoset monkeys	None
C.2	MT241	Mabtech (3520M-3-250)	IgG1	Recombinant human IL-17A	Human, rhesus and cynomolgus macaques	None
C.3	MT2770	Mabtech (3521-14-250)	IgG1	Recombinant mouse IL-17A	Mouse	None
C.4	MT504	Mabtech (3520-6-250)	IgG1	Recombinant human IL-17A	Human, rhesus and cynomolgus macaques and common marmoset monkeys	Biotin
D.1	41809	R & D Systems IC317P	IgG2b	Recombinant human IL-17A amino acids 20-155	Human	PE
D.2	41802	R & D Systems (IC3171P)	IgG1	Recombinant human IL-17A amino acids 20-155	Human	PE

All details of antibody clone, product code, immunogen, host specificity and antibody conjugate have been taken from supplier datasheets. Each antibody has been assigned a code where the capital letter denotes the commercial supplier and the integer an individual pab or a mab clone. The commercial antibodies were used in conjunction with an appropriate control antibody (see Table 2, denoted by the equivalent letter but in lowercase) for the intracellular staining protocol described in “Evaluation of commercial antibodies section”.

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