In vitro Ag restimulation. The v-src-specific interleukin (IL-12, IL-2, IFNγ, IL-10) responses were measured in chickens of the CB and CB.RI lines using the Bio-Plex system in the culture supernatants. Horizontal bars indicate the mean values. The results represent at least two independent experiments. Control (n = 5 + 5): non-restimulated control; SA-vsrc-10 (n = 5 + 5): samples restimulated with the mixture of tetramers SA-vsrcA and SA-vsrcB of immunized and non-immunized animals; SA-10 (n = 5 + 5): free SA restimulation control. Statistical significance determined by the Student’s t test: *p < 0.05; **p < 0.01; ***p < 0.001.