Characterization of Brucella abortus mutant strain Δ22915, a potential vaccine candidate

Table 2 Real-time PCR verification of differentially expressed genes in mutant strain ∆22915

Gene locus^a	Description of genes	Function^b	Fold changes (2^−∆∆Ct) ±SD
BAB_RS24460	Extracellular ligand-binding receptor	Amino acid transport and metabolism	39.08 ± 4.25
BAB_RS17405	Dihydropyrimidinase	Nucleotide transport and metabolism	20.50 ± 2.27
BAB_RS30485	Extracellular solute-binding protein family 1	Amino acid transport and metabolism	15.35 ± 1.80
BAB_RS17430	Oxidoreductase	Amino acid transport and metabolism	12.67 ± 1.25
BAB_RS31735	Amidohydrolase	Function unknown	3.63 ± 0.27
BAB_RS27765	Fumarylacetoacetate (Faa) hydrolase	Function unknown	2.80 ± 0.22
BAB_RS30280	Quinone oxidoreductase	Energy production and conversion	1.82 ± 0.20
BAB_RS30270	Abc transporter permease protein	Amino acid transport and metabolism	1.70 ± 0.24
BAB_RS26970	Flagellar basal-body rod protein	Cell motility	1.62 ± 0.31
BAB_RS18915	Gene transfer agent	Function unknown	1.55 ± 0.22
BAB_RS30285	Transcriptional regulator, GntR family	Transcription	1.52 ± 0.26
BAB_RS30275	Extracellular ligand-binding receptor	Amino acid transport and metabolism	1.37 ± 0.14
BAB_RS28745	Abc transporter permease protein	Amino acid transport and metabolism	1.37 ± 0.35
BAB_RS 28215	Transposase	Replication, recombination and repair	1.25 ± 0.23
BAB_RS27910	Transcriptional regulator	Transcription	0.81 ± 0.19
BAB_RS22920	Auxin efflux carrier	Function unknown	0.76 ± 0.11

Genes with over twofold changes levels and high probability were further validated with qRT-PCR.
^aBased on B. abortus S2308 genome (GenBank Code: NC_007618.1 and NC_007624.1).
^bThe functional categories of protein were predicted by searching through EggNOG database [33] with BLASTP.

ISSN: 1297-9716